S08-12 The ETS family transcription factor, ETV2, activates a hierarchy of endothelial and myeloid transcription factors
نویسنده
چکیده
intracellular components have been identified in both invertebrate and vertebrate, extracellular regulation that allows the morphogenesis cell mass maintaining the epithelial integrity is largely unknown. Here, we present Nectin2, a cell adhesion molecule belonging to the immunoglobulin family has an essential role in the apical constriction. Nectin2 expression is spatially and temporally regulated and its transcripts are confined to the neural plate in Xenopus neurula embryo. Depletion of Nectin2 by antisense-morpholino oligonucleotide resulted in defects in neural tube closure, while overexpression of Nectin2 in ventral ectoderm caused ectopic apical constriction. Analysis at the cellular level revealed that the cortical actin accumulation was severely affected. Interestingly, we found that the intracellular domain of Nectin2 harboring the binding site for Afadin which links Nectin2 to actin is not essential for the constricting activity. Instead, we found that N-cadherin plays a cooperative role in the apical constriction through the interaction with Nectin2. In this presentation, we will discuss about the new regulatory mechanism for apical constriction by the cell adhesion molecules.
منابع مشابه
Fli1 acts downstream of Etv2 to govern cell survival and vascular homeostasis via positive autoregulation.
RATIONALE Cardiovascular health depends on proper development and integrity of blood vessels. Ets variant 2 (Etv2), a member of the E26 transforming-specific family of transcription factors, is essential to initiate a transcriptional program leading to vascular morphogenesis in early mouse embryos. However, endothelial expression of the Etv2 gene ceases at midgestation; therefore, vascular deve...
متن کاملInduction of hematopoietic and endothelial cell program orchestrated by ETS transcription factor ER71/ETV2.
The ETS factor ETV2 (aka ER71) is essential for the generation of the blood and vascular system, as ETV2 deficiency leads to a complete block in blood and endothelial cell formation and embryonic lethality in the mouse. However, the ETV2-mediated gene regulatory network and signaling governing hematopoietic and endothelial cell development are poorly understood. Here, we map ETV2 global binding...
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متن کاملThe ETS Factor, ETV2: a Master Regulator for Vascular Endothelial Cell Development
Appropriate vessel development and its coordinated function is essential for proper embryogenesis and homeostasis in the adult. Defects in vessels cause birth defects and are an important etiology of diseases such as cardiovascular disease, tumor and diabetes retinopathy. The accumulative data indicate that ETV2, an ETS transcription factor, performs a potent and indispensable function in media...
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عنوان ژورنال:
- Mechanisms of Development
دوره 126 شماره
صفحات -
تاریخ انتشار 2009